Affiliation:
1. Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom
Abstract
ABSTRACT
Downstream elements (DSEs) with transcriptional pausing activity play an important role in transcription termination of RNA polymerase II. We have defined two such DSEs in
Schizosaccharomyces pombe
, one for the
ura4
gene and a new one in the 3′-end region of the
nmt2
gene. Although these DSEs do not have sequence homology, both are orientation specific and are composed of multiple and redundant sequence elements that work together to achieve full pausing activity. Previous studies on the
nmt1
and
nmt2
genes revealed that transcription extends several kilobases past the genes’ poly(A) sites. We show that the insertion of either DSE immediately downstream of the
nmt1
poly(A) site induces more immediate termination.
nmt2
termination efficiency can be increased by moving the DSE closer to the poly(A) site. These results suggest that DSEs may be a common feature in yeast genes.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
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