Structural Analysis of Human Immunodeficiency Virus Type 1 CRF01_AE Protease in Complex with the Substrate p1-p6-Reference-Cited by-同舟云学术

Structural Analysis of Human Immunodeficiency Virus Type 1 CRF01_AE Protease in Complex with the Substrate p1-p6

Published:2008-07 Issue:13 Volume:82 Page:6762-6766
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Bandaranayake Rajintha M.¹,Prabu-Jeyabalan Moses¹,Kakizawa Junko²,Sugiura Wataru²,Schiffer Celia A.¹

Affiliation:

1. Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 364 Plantation Street, Worcester, Massachusetts 01605

2. Laboratory of Therapeutic Research and Clinical Science, AIDS Research Center, National Institute of Infectious Diseases, 4-7-1 Gakuenn Musashimurayama, Tokyo 208-0011, Japan

Abstract

ABSTRACT The effect of amino acid variability between human immunodeficiency virus type 1 (HIV-1) clades on structure and the emergence of resistance mutations in HIV-1 protease has become an area of significant interest in recent years. We determined the first crystal structure of the HIV-1 CRF01_AE protease in complex with the p1-p6 substrate to a resolution of 2.8 Å. Hydrogen bonding between the flap hinge and the protease core regions shows significant structural rearrangements in CRF01_AE protease compared to the clade B protease structure.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.00018-08

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