Affiliation:
1. Department of Bacteriology and Immunology and Curriculum in Genetics, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27514
Abstract
We have isolated several new φX174 mutants which contain sites sensitive to restriction by
Escherichia coli.
One contains an
E. coli
15 restriction site and three are double mutants containing an
E. coli
K site as well as the
E. coli
15 site. The replicative form (RF) DNA of one of the mutants containing a K site has been shown to be restricted in spheroplasts of a K-12 strain. The infectivity of this RF, but not wild-type RF, has also been shown to be inactivated by an
E. coli
K extract and by purified K restriction enzyme in vitro. The product of the RF treated with purified K restriction enzyme in vitro is a full length linear molecule. The mutant sites have also been localized to specific regions of the φX174 genome by a fragment mapping technique, making use of specific fragments of φX174 RF DNA obtained by digestion with a specific endonuclease.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Cited by
16 articles.
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