Molecular Characterization of Baseline Enterobacterales and Pseudomonas aeruginosa Isolates from a Phase 3 Nosocomial Pneumonia (ASPECT-NP) Clinical Trial-Reference-Cited by-同舟云学术

Molecular Characterization of Baseline Enterobacterales and Pseudomonas aeruginosa Isolates from a Phase 3 Nosocomial Pneumonia (ASPECT-NP) Clinical Trial

Published:2021-02-17 Issue:3 Volume:65 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Castanheira Mariana¹^ORCID,Johnson Matthew G.²,Yu Brian²,Huntington Jennifer A.²,Carmelitano Patricia²,Bruno Christopher²,Rhee Elizabeth G.²,Motyl Mary²

Affiliation:

1. JMI Laboratories, North Liberty, Iowa, USA

2. Merck & Co., Inc., Kenilworth, New Jersey, USA

Abstract

We evaluated β-lactam-resistant baseline Enterobacterales species and Pseudomonas aeruginosa lower respiratory tract isolates collected during the ASPECT-NP phase 3 clinical trial evaluating the safety and efficacy of ceftolozane-tazobactam compared with meropenem for the treatment of nosocomial pneumonia in ventilated adults. Isolates were subjected to whole-genome sequencing, quantitative real-time PCR for quantification of the expression levels of β-lactamase and efflux pump genes, and Western blot analysis for the detection of OprD ( P. aeruginosa only).

Funder

Merck

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.02461-20

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