Purification and Characterization of the Acetone Carboxylase of Cupriavidus metallidurans Strain CH34

Author:

Rosier Caroline,Leys Natalie,Henoumont Céline,Mergeay Max,Wattiez Ruddy

Abstract

ABSTRACTAcetone carboxylase (Acx) is a key enzyme involved in the biodegradation of acetone by bacteria. Except for theHelicobacteraceaefamily, genome analyses revealed that bacteria that possess an Acx, such asCupriavidus metalliduransstrain CH34, are associated with soil. The Acx of CH34 forms the heterohexameric complex α2β2γ2and can carboxylate only acetone and 2-butanone in an ATP-dependent reaction to acetoacetate and 3-keto-2-methylbutyrate, respectively.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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