Effects of glucose, growth temperature, and pH on listeriolysin O production in Listeria monocytogenes

Author:

Datta A R1,Kothary M H1

Affiliation:

1. Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.

Abstract

Expression of listeriolysin O of Listeria monocytogenes as a function of different growth conditions was studied by performing a direct hemolysin assay, immunoblotting experiments, and an enzyme-linked immunosorbent assay. Expression of listeriolysin O was reduced at a lower growth temperatures (26 degrees C) and at higher glucose concentrations (> or = 0.3%) in the growth media. The effect of glucose appeared to be due to a change in the pH of the growth media.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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