Affiliation:
1. Center for Biological Resource Recovery and Departments of Microbiology and Biochemistry, University of Georgia, Athens, Georgia 30602
Abstract
Autolysis was induced to form stable, cell wall-free cells of
Clostridium thermohydrosulfuricum
JW102 and
Thermoanaerobacter ethanolicus
JW200, using a complex medium containing glycine (0.4% wt/vol) and/or sucrose or glycerol (10% wt/vol) at an optimum temperature of 64°C. Autoplasts of both bacteria were grown as L-phase colonies on solid medium; more than 50% of these colonies regenerated to the walled form during prolonged incubation. The removal of the cell wall was confirmed by electron microscopy.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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