Fusarium poae and Fusarium crookwellense , Fungi Responsible for the Natural Occurrence of Nivalenol in Hokkaido

Author:

Sugiura Yoshitsugu1,Fukasaku Kazuaki1,Tanaka Toshitsugu1,Matsui Yukio1,Ueno Yoshio1

Affiliation:

1. Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, 12, Ichigaya Funagawara-Machi, Shinjuku-Ku, Tokyo 162, Research Institute for Biosciences, Science University of Tokyo, 2669, Yamazaki, Noda-Shi, Chiba 2782, Public Health Research Institute of Kobe City, 4-6, Minatojima-Nakamachi, Chuo-Ku, Kobe 6503, and Department of Dairy Science, College of Dairying, 582-1, Bunkyodai-Midori-Machi, Ebetsu, Hokkaido 069, 4 Japan

Abstract

To determine the reasons for the natural occurrence of nivalenol in the northernmost area of Japan, scabby wheat was harvested from 19 crop fields in Hokkaido. Mycological surveys and analysis for mycotoxin contamination were performed. Among 13 wheat grain samples harvested in seven locations, 9, 2, and 6 samples were positive for deoxynivalenol, nivalenol, and zearalenone, respectively, at levels ranging from 0.03 to 1.28 μg/g, 0.04 to 1.22 μg/g, and 2 to 25 ng/g, respectively. The predominant Fusarium species of the scabby wheat examined were F. sporotrichioides, F. avenaceum, F. poae , and F. crookwellense. Fifteen of 48 F. poae isolates and all four F. crookwellense isolates were screened for the production of seven derivatives of trichothecenes and zearalenone respectively, on rice culture. One isolate of F. poae produced diacetoxyscirpenol alone (4.3 μg/g); seven produced nivalenol (1.3 to 23.8 μg/g), 4-acetylnivalenol (0.1 to 4.6 μg/g), and diacetoxyscirpenol (0.9 to 99.5 μg/g); and five produced nivalenol alone (0.4 to 3.5 μg/g). The remaining two isolates produced no trichothecenes. Zearalenone production was not found in any isolate of F. poae tested. All isolates of F. crookwellense produced nivalenol (0.9 to 22.5 μg/g), 4-acetylnivalenol (0.5 to 25.0 μg/g), and zearalenone (1.4 to 162.5 μg/g). From these results, it is apparent that deoxynivalenol and zearalenone, and occasionally nivalenol, occur naturally throughout Hokkaido, and it is suggested that nivalenol-producing F. poae and F. crookwellense strains are responsible for the natural contamination with nivalenol found in the northernmost area of Japan. Furthermore, it was found for the first time that several isolates of F. poae distributed in Hokkaido possessed the ability to produce both type A and type B trichothecenes.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference28 articles.

1. Booth C. 1971. The genus Fusarium. Commonwealth Mycological Institute Kew Surrey England.

2. Characterization, geographic distribution and ecology of Fusarium crookwellense sp. nov;Burgess L. W.;Trans. Br. Mycol. Soc.,1982

3. Variation in the cultural characteristics and pathogenicity of Fusarum avenaceum and F. arthrosporioides;Cormack M. W.;Can. J. Bot.,1950

4. Frisvad J. C. and R. A. Samson. 1991. Filamentous fungi in foods and feeds: ecology spoilage and mycotoxin production p. 31-68. In D. K. Arora K. G. Mukerji and E. H. Marth. (ed.) Handbook of applied mycology vol. 3: foods and feeds. Marcel Dekker Inc. New York.

5. Formation of fusarenone X, nivalenol, zearalenone, 8-trans-zearalenol, 3-trans-zearalenol, and fusarin C by Fusanum crookwellense;Golinski P.;Appl. Environ. Microbiol.,1988

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