Affiliation:
1. Centro de Recursos Microbiológicos, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal
Abstract
ABSTRACT
Rhodosporidium toruloides
is a heterothallic, bipolar, red yeast that belongs to the
Sporidiobolales
, an order within a major lineage of basidiomycetes, the
Pucciniomycotina
. In contrast to other basidiomycetes, considerably less is known about the nature of the mating type (
MAT
) loci that control sexual reproduction in this lineage. Three genes (
RHA1, RHA2
, and
RHA3
) encoding precursors of the
MAT A1
pheromone (rhodotorucine
A
) were previously identified and formed the basis for a genome walking approach that led to the identification of additional
MAT
genes in complementary mating strains of
R. toruloides
. Two mating type-specific alleles encoding a p21-activated kinase (PAK; Ste20 homolog) were found between the
RHA2
and
RHA3
genes, and identification in
MAT A2
strains of a gene encoding a presumptive pheromone precursor enabled prediction of the structure of rhodotorucine
a
. In addition, a putative pheromone receptor gene (
STE3
homolog) was identified upstream of
RHA1
. Analyses of genomic data from two closely related species,
Sporobolomyces roseus
and
Sporidiobolus salmonicolor
, identified syntenic regions that contain homologs of all the above-mentioned genes. Notably, six novel pheromone precursor genes were uncovered, which encoded, similarly to the
RHA
genes, multiple tandem copies of the peptide moiety. This suggests that this structure, which is unique among fungal lipopeptide pheromones, seems to be prevalent in red yeasts. Species comparisons provided evidence for a large, multigenic
MAT
locus structure in the
Sporidiobolales
, but no putative homeodomain transcription factor genes (which are present in all basidiomycetous
MAT
loci characterized thus far) could be found in any of the three species in the vicinity of the
MAT
genes identified.
Publisher
American Society for Microbiology
Subject
Molecular Biology,General Medicine,Microbiology
Cited by
35 articles.
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