Affiliation:
1. Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104
Abstract
The rate of protein synthesis by
Escherichia coli
markedly decreased within 1 min after phage T4 infection, whereas a complete cessation of protein synthesis was observed within at least 25 sec after T4 ghost infection. The cellular level of amino acids and aminoacyl-transfer ribonucleic acid (tRNA) did not change drastically upon infection with ghosts, indicating that the inhibition of protein synthesis took place at a step(s) beyond aminoacyl-tRNA formation. The host messenger RNA remained intact and still bound to ribosomes shortly after ghost infection. Kinetic studies of the effect of ghosts on host protein synthesis revealed that nascent peptide chains on ribosomes were not released upon ghost infection.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
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