The Properties of a tRNA-Specific Adenosine Deaminase from Drosophila melanogaster Support an Evolutionary Link between Pre-mRNA Editing and tRNA Modification

Author:

Keegan Liam P.1,Gerber André P.2,Brindle Jim1,Leemans Ronny3,Gallo Angela1,Keller Walter2,O'Connell Mary A.1

Affiliation:

1. MRC Human Genetics Unit, Western General Hospital, Edinburgh EH4 2XU, United Kingdom, 1 and

2. Department of Cell Biology, Biozentrum of the University of Basel, CH-4056 Basel, 2 and

3. Zoological Institute, University of Basel, CH-4051 Basel, 3 Switzerland

Abstract

ABSTRACT Pre-mRNA editing involving the conversion of adenosine to inosine is mediated by adenosine deaminases that act on RNA (ADAR1 and ADAR2). ADARs contain multiple double-stranded RNA(dsRNA)-binding domains in addition to an adenosine deaminase domain. An adenosine deaminase acting on tRNAs, scTad1p (also known as scADAT1), cloned from Saccharomyces cerevisiae has a deaminase domain related to the ADARs but lacks dsRNA-binding domains. We have identified a gene homologous to scADAT1 in the region of Drosophila melanogaster Adh chromosome II. Recombinant Drosophila ADAT1 (dADAT1) has been expressed in the yeast Pichia pastoris and purified. The enzyme has no activity on dsRNA substrates but is a tRNA deaminase with specificity for adenosine 37 of insect alanine tRNA. dADAT1 shows greater similarity to vertebrate ADARs than to yeast Tad1p, supporting the hypothesis of a common evolutionary origin for ADARs and ADATs. dAdat1 transcripts are maternally supplied in the egg. Zygotic expression is widespread initially and later concentrates in the central nervous system.

Publisher

American Society for Microbiology

Subject

Cell Biology,Molecular Biology

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