Use of the BacT/Alert MB Mycobacterial Blood Culture System for Detection of Mycobacteria in Sterile Body Fluids Other than Blood

Author:

Mattei Romano1,Savarino Arnaldo1,Fabbri Marco2,Moneta Sara3,Tortoli Enrico4

Affiliation:

1. Clinical Laboratory

2. Blood Bank

3. Department of Infectious Diseases, Campo di Marte Hospital, Lucca 55100

4. Regional Reference Center for Mycobacteria, Microbiology and Virology Laboratory, Careggi Hospital, Florence 50134, Italy

Abstract

The definitive diagnosis of extrapulmonary tuberculosis is made by a positive body fluid culture result. Conventional culture methods require centrifugation or filtration of body fluid (peritoneal, pleural, synovial, or pericardial fluid) in order to improve the sensitivity. The aim of the present study was to evaluate the feasibility of the direct inoculation, at the patient's bedside, of up to 5 ml of uncentrifuged fluid onto BacT/Alert MB culture bottles (bioMérieux, Durham, NC).

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference7 articles.

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3. Goel, M. M., V. Ranjan, T. N. Dhole, A. N. Srivastava, A. Mehrotra, M. R. Kushwaha, and A. Jain. 2001. Polymerase chain reaction vs. conventional diagnosis in fine needle aspirates of tuberculous lymph nodes. Acta Cytol. 43:333-340.

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1. Preanalytic Considerations;Quick Guide to Body Fluid Testing;2023

2. Rapid Identification of Microorganisms from Sterile Body Fluids by Use of FilmArray;Journal of Clinical Microbiology;2014-12-17

3. Estrategias diagnósticas y terapéuticas en las enfermedades infecciosas;Medicine - Programa de Formación Médica Continuada Acreditado;2014-05

4. EARLY DIAGNOSIS OF TUBERCULOUS PERITONITIS BY NESTED PCR AND AUTOMATED CULTURE TECHNIQUE;Journal of Evolution of Medical and Dental Sciences;2012-11-13

5. Rapid culture diagnosis of tuberculous lymphadenitis from a tertiary care centre in an endemic nation: Potential and pitfalls;Indian Journal of Medical Microbiology;2012-07

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