Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome-Reference-Cited by-同舟云学术

Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome

Published:1989-02 Issue:2 Volume:171 Page:1214-1218
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Tabata S¹,Higashitani A¹,Takanami M¹,Akiyama K¹,Kohara Y¹,Nishimura Y¹,Nishimura A¹,Yasuda S¹,Hirota Y¹

Affiliation:

1. Department of Biology, Faculty of Science, Nagoya University, Japan.

Abstract

A cosmid library of the Escherichia coli K-12 W3110 chromosome was constructed in which clones were assigned to locations on the chromosome map by hybridization and genetic marker complementation tests. Approximately 70% of the genome was represented by this library. The identified clones can be maintained in the homologous system and would facilitate genetic studies of E. coli.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.171.2.1214-1218.1989

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