Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome

Author:

Tabata S1,Higashitani A1,Takanami M1,Akiyama K1,Kohara Y1,Nishimura Y1,Nishimura A1,Yasuda S1,Hirota Y1

Affiliation:

1. Department of Biology, Faculty of Science, Nagoya University, Japan.

Abstract

A cosmid library of the Escherichia coli K-12 W3110 chromosome was constructed in which clones were assigned to locations on the chromosome map by hybridization and genetic marker complementation tests. Approximately 70% of the genome was represented by this library. The identified clones can be maintained in the homologous system and would facilitate genetic studies of E. coli.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference49 articles.

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