Cell Wall Turnover at the Hemispherical Caps of Bacillus subtilis

Author:

Fan David P.1,Beckman Bruce E.1,Beckman Mary M.1

Affiliation:

1. Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55101

Abstract

Cell walls made by Bacillus subtilis bacteria grown in D 2 O medium have buoyant densities in CsCl which are different from walls made by cells grown in H 2 O medium. Cell wall turnover was studied by measuring the change in wall buoyant density after a B. subtilis culture was shifted from growth in D 2 O medium to aeration in H 2 O medium. Walls from the hemispherical caps were isolated after preferential digestion of wall from the cylindrical regions using the B. subtilis autolytic amidase. The walls from the polar regions were found to turn over extensively.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference7 articles.

1. Anderson N. G. and N. L. Anderson. 1970. Density at 25 C of CsCl solution as a function of refractive index p. J292-J296. In H. A. Sober (ed.) CRC handbook of biochemistry 2nd ed. Chemical Rubber Co. Cleveland Ohio.

2. Symposium on the fine structure and replication of bacteria and their parts. III. Bacterial cell-wall replication followed by immunofluorescence;Cole R. M.;Bacteriol. Rev.,1965

3. Cell wall binding properties of the Bacillus subtilis autolysin(s);Fan D. P.;J. Bacteriol.,1970

4. Autolysin(s) of Bacillus subtilis as dechaining enzyme;Fan D. P.;J. Bacteriol.,1970

5. Structural difference between walls from hemispherical caps and partial septa of Bacillus subtilis;Fan D. P.;J. Bacteriol.,1973

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