Sequential Recruitment of the Endoplasmic Reticulum and Chloroplasts for Plant Potyvirus Replication

Author:

Wei Taiyun12,Huang Tyng-Shyan12,McNeil Jamie1,Laliberté Jean-François3,Hong Jian4,Nelson Richard S.5,Wang Aiming12

Affiliation:

1. Southern Crop Protection and Food Research Centre, Agriculture and Agri-Food Canada, 1391 Sandford Street, London, Ontario N5V 4T3, Canada

2. Department of Biology, The University of Western Ontario, 1151 Richmond Street, London, Ontario N6A 5B7, Canada

3. Institut Armand-Frappier, Institut National de la Recherche Scientifique, 531 Boulevard des Prairies, Laval, Québec H7V 1B7, Canada

4. Institute of Biotechnology, Zhejiang University, Hangzhou 310029, People's Republic of China

5. Plant Biology Division, Samuel Roberts Noble Foundation, Inc., 2510 Sam Noble Parkway, Ardmore, Oklahoma 73401

Abstract

ABSTRACT The replication of positive-strand RNA viruses occurs in cytoplasmic membrane-bound virus replication complexes (VRCs). Depending on the virus, distinct cellular organelles such as the endoplasmic reticulum (ER), chloroplast, mitochondrion, endosome, and peroxisome are recruited for the formation of VRC-associated membranous structures. Previously, the 6,000-molecular-weight protein (6K) of plant potyviruses was shown to be an integral membrane protein that induces the formation of 6K-containing membranous vesicles at endoplasmic reticulum (ER) exit sites for potyvirus genome replication. Here, we present evidence that the 6K-induced vesicles predominantly target chloroplasts, where they amalgamate and induce chloroplast membrane invaginations. The vesicular transport pathway and actomyosin motility system are involved in the trafficking of the 6K vesicles from the ER to chloroplasts. Viral RNA, double-stranded RNA, and viral replicase components are concentrated at the 6K vesicles that associate with chloroplasts in infected cells, suggesting that these chloroplast-bound 6K vesicles are the site for potyvirus replication. Taken together, these results suggest that plant potyviruses sequentially recruit the ER and chloroplasts for their genome replication.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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