Production of Capsular Polysaccharide of Streptococcus pneumoniae Type 14 and Its Purification by Affinity Chromatography-Reference-Cited by-同舟云学术

Production of Capsular Polysaccharide of Streptococcus pneumoniae Type 14 and Its Purification by Affinity Chromatography

Published:2001-02 Issue:2 Volume:67 Page:969-971
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Suárez Norma¹,Fraguas Laura Franco²,Texeira Esther³,Massaldi Hugo³,Batista-Viera Francisco²,Ferreira Fernando¹

Affiliation:

1. Laboratorio de Carbohidratos y Glicoconjugados, Departamento de Quimica Orgánica, Facultad de Quı́mica,1 and

2. Facultad de Quı́mica, Cátedra de Bioquı́mica, CC 1157,2 Montevideo, Uruguay

3. Departamento de Desarrollo y Producción, Facultad de Medicina,3 Instituto de Higiene, C.P. 11600, and

Abstract

ABSTRACT We describe a rapid and efficient method for producing the capsular polysaccharide of Streptococcus pneumoniae by fermentation on tryptic soy broth and purification of this compound by using immobilized soybean lectin as an affinity adsorbent. In principle, the same strategy can be used to produce purified capsular polysaccharides from other streptococcal serotypes by selecting the appropriate lectin adsorbents.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.67.2.969-971.2001

Reference13 articles.

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