Author:
Harms N,de Vries G E,Maurer K,Hoogendijk J,Stouthamer A H
Abstract
A genomic clone bank of Paracoccus denitrificans DNA has been constructed in the expression vector set pEX1, pEX2, and pEX3. Screening of this clone bank with antibodies raised against P. denitrificans methanol dehydrogenase resulted in the isolation of a clone, pNH3, that synthesized methanol dehydrogenase cross-reactive proteins. The nucleotide sequence of the P. denitrificans DNA fragment inserted in this clone has been determined and shown to contain the full methanol dehydrogenase structural gene. DNA cross-hybridization was found with DNA fragments which have been reported to contain the methanol dehydrogenase structural genes from Methylobacterium sp. strain AM1 and Methylobacterium organophilum.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference31 articles.
1. Construction of broadhost-range cloning vectors: identification of genes necessary for growth of Methylobacterium organophilum on methanol;Allen L. N.;J. Bacteriol.,1985
2. Anthony C. 1982. The biochemistry of methylotrophs. Academic Press Inc. (London) Ltd. London.
3. Aerobic and anaerobic growth of Paracoccus denitrificans on methanol;Bamforth C. W.;Arch. Micobiol.,1978
4. The in vitro transcriptiontranslation of DNA and RNA templates by extracts of Rhodopseudomonas sphaeroides;Chory J.;J. Biol. Chem.,1982
5. Purification and characterization of a complex between cloacin and its immunity protein isolated from Enterobacter cloacae (Clo Df 13);de Graaf F. K.;Eur. J. Biochem.,1977
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