Molecular Method for Identification of Rickettsia Species in Clinical and Environmental Samples-Reference-Cited by-同舟云学术

Molecular Method for Identification of Rickettsia Species in Clinical and Environmental Samples

Published:2006-12 Issue:12 Volume:44 Page:4572-4576
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Jado Isabel¹²,Escudero Raquel¹,Gil Horacio¹,Jiménez-Alonso María Isabel¹,Sousa Rita³,García-Pérez Ana L.⁴,Rodríguez-Vargas Manuela¹,Lobo Bruno¹,Anda Pedro¹

Affiliation:

1. Laboratorio de Espiroquetas y Patógenos Especiales, Servicio de Bacteriología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain

2. Unidad de Alertas y Emergencias, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain

3. Centro de Estudos de Vectores e Doenças Infecciosas (CEVDI), Instituto Nacional de Saude, Lisboa, Portugal

4. Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER), Derio, Vizcaya, Spain

Abstract

ABSTRACT We present a PCR method targeting the 23S-5S internal transcribed spacer coupled with reverse line blotting that allows Rickettsia species detection and identification in a single step. The method is highly sensitive and specific in identifying Rickettsia species from both patient and environmental samples. The generic approach used allowed us to identify new pathogens.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01227-06

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