Effects of Microplate Type and Broth Additives on Microdilution MIC Susceptibility Assays-Reference-Cited by-同舟云学术

Effects of Microplate Type and Broth Additives on Microdilution MIC Susceptibility Assays

Published:2019-01 Issue:1 Volume:63 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Kavanagh Angela¹,Ramu Soumya¹,Gong Yujing¹,Cooper Matthew A.¹,Blaskovich Mark A. T.¹^ORCID

Affiliation:

1. Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Queensland, Australia

Abstract

The determination of antibiotic potency against bacterial strains by assessment of their minimum inhibitory concentration normally uses a standardized broth microdilution assay procedure developed more than 50 years ago. However, certain antibiotics require modified assay conditions in order to observe optimal activity.

Funder

HHS | National Institutes of Health

Wellcome Trust

Department of Health | National Health and Medical Research Council

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.01760-18

Reference75 articles.

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2. Clinical and Laboratory Standards Institute. 2018. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard, 11th ed. CLSI document M07-A11. Clinical and Laboratory Standards Institute, Wayne, PA.

3. CLSI. 2018. Performance standards for antimicrobial susceptibility testing; 22nd informational supplement, 28th ed. CLSI document M100-S28. Clinical and Laboratory Standards Institute, Wayne, PA.

4. Determination of minimum inhibitory concentrations

5. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances

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