Author:
Van Gijsegem F,Toussaint A
Abstract
Using the RP4::mini-Mu pULB113 plasmid, an RP4 derivative carrying a deleted Mu prophage which allows the plasmid to pick up any chromosomal DNA segment to form R' plasmids, we cloned all of the genes of Erwinia carotovora involved in the catabolism of the hexuronates and in the transport of these substrates. With the R' plasmids we isolated, we performed complementation analysis and found that, in the Erwinia carotovora strain we used, the genes involved in the catabolism of the hexuronates are clustered in four regions of the chromosome. This genetic organization is compared with that of Escherichia coli K-12.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference31 articles.
1. Enzymes of glucuronic and galacturonic acid metabolism in bacteria;Ashwell G.;Methods Enzymol.,1962
2. Linkage map of Escherichia coli K-12, edition 6;Bachman G. J.;Microbiol. Rev.,1980
3. A restriction endonuclease analysis of the bacterial plasmid controlling the EcoRl restriction and modification of DNA;Betlach M. D.;Fed. Proc.,1976
4. Cabezas de Herrera E. and E. Sanchez Maeso. 1978. Isolation purification and estimation of the different pectic enzymes of Erwinia carotovora p. 617-629. In Proceedings of the Fourth International Conference on Plant Pathogenic Bacteria Angers France.
5. Phage Mu-1 mediated transposition: a tool to study the organization of ribosomal protein genes in E. coli;Cabezon T.;Mol. Gen. Genet.,1978
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