Structure-Function Analysis of Escherichia coli MnmG (GidA), a Highly Conserved tRNA-Modifying Enzyme-Reference-Cited by-同舟云学术

Structure-Function Analysis of Escherichia coli MnmG (GidA), a Highly Conserved tRNA-Modifying Enzyme

Published:2009-12-15 Issue:24 Volume:191 Page:7614-7619
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Shi Rong¹,Villarroya Magda²,Ruiz-Partida Rafael²,Li Yunge³,Proteau Ariane¹,Prado Silvia²,Moukadiri Ismaïl²,Benítez-Páez Alfonso²,Lomas Rodrigo²,Wagner John³,Matte Allan³,Velázquez-Campoy Adrián⁴,Armengod M.-Eugenia²,Cygler Miroslaw¹³

Affiliation:

1. Department of Biochemistry, McGill University, Montreal, Quebec, Canada

2. Laboratorio de Genética Molecular, Centro de Investigación Príncipe Felipe, Avenida Autopista del Saler, 16-3, 46012 Valencia, Spain

3. Biotechnology Research Institute, NRC, 6100 Royalmount Avenue, Montreal, Quebec H4P 2R2, Canada

4. Institute of Biocomputation and Physics of Complex Systems (BIFI), Universidad de Zaragoza, Saragossa, Spain, and Fundación Aragón I+D (ARAID-BIFI), Diputación General de Aragón, Aragon, Spain

Abstract

ABSTRACT The MnmE-MnmG complex is involved in tRNA modification. We have determined the crystal structure of Escherichia coli MnmG at 2.4-Å resolution, mutated highly conserved residues with putative roles in flavin adenine dinucleotide (FAD) or tRNA binding and MnmE interaction, and analyzed the effects of these mutations in vivo and in vitro. Limited trypsinolysis of MnmG suggests significant conformational changes upon FAD binding.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.00650-09

Reference9 articles.

1. Björk, G. R. 1994. Biosynthesis and function of modified nucleosides, p. 165-205. In D. Soll and U. L. RajBhandary (ed.), tRNA: structure, biosynthesis, and function. American Society for Microbiology, Washington, DC.

2. Czerwoniec, A., S. Dunin-Horkawicz, E. Purta, K. H. Kaminska, J. M. Kasprzak, J. M. Bujnicki, H. Grosjean, and K. Rother. 2009. MODOMICS: a database of RNA modification pathways. 2008 update. Nucleic Acids Res. 37 : D118-D121.

3. Iwasaki, W., H. Miyatake, and K. Miki. 2005. Crystal structure of the small form of glucose-inhibited division protein A from Thermus thermophilus HB8. Proteins 61 : 1121-1126.

4. Martinez-Vicente, M., L. Yim, M. Villarroya, M. Mellado, E. Perez-Paya, G. R. Bjork, and M. E. Armengod. 2005. Effects of mutagenesis in the switch I region and conserved arginines of Escherichia coli MnmE protein, a GTPase involved in tRNA modification. J. Biol. Chem. 280 : 30660-30670.

5. Meyer, S., A. Scrima, W. Versées, and A. Wittinghofer. 2008. Crystal structures of the conserved tRNA-modifying enzyme GidA: implications for its interaction with MnmE and substrate. J. Mol. Biol. 380 : 532-547.

Cited by 43 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Genomic analysis of the degradation gene clusters and the parABS system in Afipia sp. strain DD3 capable of utilizing 2,4-dichlorophenoxyacetic acid;The Microbe;2024-09

2. Insights into the Mechanism of Installation of 5-Carboxymethylaminomethyl Uridine Hypermodification by tRNA-Modifying Enzymes MnmE and MnmG;Journal of the American Chemical Society;2023-12-05

3. VirB, a key transcriptional regulator of Shigella virulence, requires a CTP ligand for its regulatory activities;mBio;2023-10-31

4. VirB, a key transcriptional regulator ofShigellavirulence, requires a CTP ligand for its regulatory activities;2023-05-17

5. Transfer RNA Modification Enzymes with a Thiouridine Synthetase, Methyltransferase and Pseudouridine Synthase (THUMP) Domain and the Nucleosides They Produce in tRNA;Genes;2023-01-31