Isolation of Gregatin A from Phialophora gregata by Preparative High-Pressure Liquid Chromatography

Author:

Taylor Scott L.1,Peterson Robert E.1,Gray Lynn E.1

Affiliation:

1. Northern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604, 1 and Department of Plant Pathology, Agricultural Research Service, U.S. Department of Agriculture, Urbana, Illinois 618012

Abstract

A method was developed for the production and purification of gregatin A from Phialophora gregata NRRL 13198 cultured on rice at 20°C for 28 days. Liquid extraction followed by high-pressure liquid chromatography afforded 247.0 mg of crystalline gregatin A per kg of rice.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference6 articles.

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2. Gams W. 1971. Cephalosporium-artige Schimmelpilze (Hyphomycetes). Gustav Fischer Verlag Stuttgart Federal Republic of Germany. NOTES 1329

3. Effect of Cephalosporium gregatum on soybean yield;Gray L. E.;Plant Dis. Rep.,1972

4. The incidence, development, and yield effects of Cephalosporium gregatum on soybeans in Illinois;Gray L. E.;Plant Dis. Rep.,1973

5. Isolation of phytotoxic substances produced by Cephalosporium gregatum Allington and Chamberlain;Kobayashi K.;Tetrahedron Lett.,1975

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