Development of Specific Nested Oligonucleotide PCR Primers for the Streptococcus iniae 16S-23S Ribosomal DNA Intergenic Spacer-Reference-Cited by-同舟云学术

Development of Specific Nested Oligonucleotide PCR Primers for the Streptococcus iniae 16S-23S Ribosomal DNA Intergenic Spacer

Published:1998-09 Issue:9 Volume:36 Page:2778-2781
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Berridge Brian R.¹,Fuller Jeffrey D.²,de Azavedo Joyce²,Low Donald E.²,Bercovier Herve³,Frelier Paul F.¹

Affiliation:

1. Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas1;

2. Department of Microbiology, University of Toronto, and Mount Sinai and Princess Margaret Hospitals, Toronto, Ontario, Canada2; and

3. Department of Clinical Microbiology, The Hebrew University- Hadassah Medical School, Jerusalem, Israel3

Abstract

ABSTRACT Streptococcus iniae is a cause of septicemia, meningoencephalitis, and death in farmed fish and of cellulitis in human beings. A set of nested oligonucleotide PCR primers that specifically amplified a 373-bp subunit from a variety of clinical isolates from farmed fish and human patients were constructed from a 524-bp consensus sequence of the S. iniae 16S-23S ribosomal DNA intergenic spacer.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.36.9.2778-2781.1998

Reference22 articles.

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5. Restriction fragment length polymorphisms of 16S rDNA and of whole rRNA genes (ribotyping) of Streptococcus iniae strains from the United States and Israel;Eldar A.;FEMS Microbiol. Lett.,1997

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