Cat Scratch Disease: the Rare Role of Afipia felis

Author:

Giladi Michael1,Avidor Boaz1,Kletter Yehudith1,Abulafia Suzy1,Slater Leonard N.2,Welch David F.3,Brenner Don J.4,Steigerwalt Arnold G.4,Whitney Anne M.4,Ephros Moshe5

Affiliation:

1. The Bernard Pridan Laboratory for Molecular Biology of Infectious Diseases, Ichilov Hospital, Tel Aviv Sourasky Medical Center, Tel Aviv,1 and

2. University of Oklahoma, Health Sciences Center, Oklahoma City, Oklahoma2;

3. Laboratory Corporation of America, and Department of Pathology, University of Texas, Southwestern Medical Center, Dallas, Texas3; and

4. Meningitis and Special Pathogens Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia4

5. the Department of Pediatrics, Carmel Medical Center, Haifa,5 Israel;

Abstract

ABSTRACT Since its isolation in 1988, Afipia felis has been associated with cat scratch disease (CSD) in only one report and its role in CSD has been questioned. We have cultured A. felis from a lymph node of a patient with CSD. 16S rRNA gene sequencing, DNA relatedness studies, fatty acid analysis, and PCR of the A. felis ferredoxin gene showed that the isolate is identical to the previously reported A. felis isolate. To determine the role of A. felis in CSD, PCR of the 16S rRNA gene followed by hybridizations with specific probes were performed with lymph node specimens from CSD patients. All 32 specimens tested positive for Bartonella henselae and negative for A. felis . We conclude that A. felis is a rare cause of CSD. Diagnostic tests not conducive to the identification of A. felis might cause the diagnosis of CSD due to A. felis to be missed.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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