Affiliation:
1. Division of Biological Sciences
2. Center for Biomolecular Structure and Dynamics, The University of Montana, Missoula, Montana 59812
Abstract
ABSTRACT
We have demonstrated that
rpsL
, encoding the S12 protein of the small ribosomal subunit, can be used as a counterselectable marker in
Borrelia burgdorferi
, the causative agent of Lyme disease. Mutations in
rpsL
confer streptomycin resistance. Streptomycin susceptibility is dominant in an
rpsL
merodiploid, and streptomycin selects for the loss of wild-type
rpsL
carried in
trans
. This is the first description of a counterselectable marker in
B. burgdorferi
.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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5. Efficient Targeted Mutagenesis in
Borrelia burgdorferi
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