Diagnosis of Enterocytozoon bieneusi by PCR in Stool Samples Eluted from Filter Paper Disks-Reference-Cited by-同舟云学术

Diagnosis of Enterocytozoon bieneusi by PCR in Stool Samples Eluted from Filter Paper Disks

Published:2000-05 Issue:3 Volume:7 Page:504-506
ISSN:1071-412X
Container-title:Clinical Diagnostic Laboratory Immunology
language:en
Short-container-title:Clin Diagn Lab Immunol

Author:

Carnevale Silvana¹,Velásquez Jorge N.¹,Labbé Jorge H.¹,Chertcoff Agustín¹,Cabrera Marta G.¹,Rodríguez Mónica I.¹

Affiliation:

1. Departamento de Parasitologı́a, Instituto Nacional de Microbiologı́a “Dr. Carlos G. Malbrán,” and Hospital Municipal de Infecciosas “Dr. Francisco J. Muñiz,” Buenos Aires, Argentina

Abstract

ABSTRACT We report a PCR-based assay for the detection of Enterocytozoon bieneusi . We extracted DNA from feces which had been applied to filter paper disks and evaluated four preserving solutions. Infected specimens were identified by electrophoresis of amplicons from concentrated formalin-fixed samples and unconcentrated fresh feces. Our findings demonstrate that this methodology is effective for sample collection, mailing, and diagnosis of this pathogen.

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CDLI.7.3.504-506.2000

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