Affiliation:
1. Communicable Disease Center, U.S. Public Health Service, U.S. Department of Health, Education, and Welfare, Atlanta, Georgia
Abstract
A comparison was made of (NH
4
)
2
SO
4
, HCl, ethodin, and ethanol for fractionation of rabbit antiserum prior to conjugation with fluorescein isothiocyanate. Fractionation with the salt was found to be the method of choice from the standpoints of simplicity and recovery of antibody effective in conjugates prepared from the fractions. Effects of pH, temperature, dye-protein ratio, and molarity and type of buffer upon conjugation were studied. These technical factors were adjusted to produce conjugates for
Corynebacterium diphtheriae
which possessed higher specific titers than did reagents obtained by previously employed techniques.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference14 articles.
1. Localization of antigen in tissue cells. II. Improvements in a method for the detection of antigen by means of fluorescent antibody;COONS A. H.;J. Exptl. Mled.,1950
2. Fluorescent antibody technic for serodiagnosis of Trypanosoma cruzi infection;FIFE E. H.;Proc. Soc. Exptl. Biol. Med.,1959
3. A comparison of fluorescein-labeled -y-globulins purified by Rivanol and DEAE chromatography;FROMMHAGEN L. H.;J. Immunol.,1963
4. The staining properties of human serum proteins conjugated with purified fluorescein isothiocyanate;FROMMHAGEN L. H.;J. Immunol.,1962
5. Determination of serum proteins by means of the biuret reaction;GORNALL A. G.;J. Biol. Chem.,1949
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