G→A Hypermutation in Protease and Reverse Transcriptase Regions of Human Immunodeficiency Virus Type 1 Residing in Resting CD4 + T Cells In Vivo-Reference-Cited by-同舟云学术

G→A Hypermutation in Protease and Reverse Transcriptase Regions of Human Immunodeficiency Virus Type 1 Residing in Resting CD4 + T Cells In Vivo

Published:2005-02 Issue:3 Volume:79 Page:1975-1980
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Kieffer Tara L.¹,Kwon Patty¹,Nettles Richard E.¹,Han Yefei¹,Ray Stuart C.¹,Siliciano Robert F.¹²

Affiliation:

1. Department of Medicine, Johns Hopkins University School of Medicine

2. Howard Hughes Medical Institute, Baltimore, Maryland

Abstract

ABSTRACT In vitro studies have shown that the host cytidine deaminase APOBEC3G causes lethal hypermutation in human immunodeficiency virus type 1 reverse transcripts unless its incorporation into virions is blocked by Vif. By examining stably archived sequences in resting CD4 + T cells, we show that hypermutation occurs in most if not all infected individuals. Hypermutated sequences comprised >9% of archived species in resting CD4 + T cells but were not found in plasma virus. Mutations occurred in predicted contexts, with notable hotspots. Thus, defects in Vif function in vivo give rise to hypermutated viral genomes that can be integrated but do not produce progeny viruses.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.79.3.1975-1980.2005

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