A bacteriophage T4 gene which functions to inhibit Escherichia coli Lon protease

Author:

Skorupski K1,Tomaschewski J1,Rüger W1,Simon L D1

Affiliation:

1. Waksman Institute of Microbiology, Rutgers, State University of New Jersey, Piscataway 08854.

Abstract

A bacteriophage T4 gene which functions to inhibit Escherichia coli Lon protease has been identified. This pin (proteolysis inhibition) gene was selected for its ability to support plaque formation by a lambda Ots vector at 40 degrees C. Southern blot experiments indicated that this T4 gene is included within the 4.9-kilobase XbaI fragment which contains gene 49. Subcloning experiments showed that T4 gene 49.1 (designated pinA) is responsible for the ability of the Ots vector to form plaques at 40 degrees C. Deficiencies in Lon protease activity are the only changes known in E. coli that permit lambda Ots phage to form plaques efficiently at 40 degrees C. lon+ lysogens of the lambda Ots vector containing pinA permitted a lambda Ots phage to form plaques efficiently at 40 degrees C. Furthermore, these lysogens, upon comparison with similar lysogens lacking any T4 DNA, showed reduced levels of degradation of puromycyl polypeptides and of canavanyl proteins. The lon+ lysogens that contained pinA exhibited other phenotypic characteristics common to lon strains, such as filamentation and production of mucoid colonies. Levels of degradation of canavanyl proteins were essentially the same, however, in null lon lysogens which either contained or lacked pinA. We infer from these data that the T4 pinA gene functions to block Lon protease activity; pinA does not, however, appear to block the activity of proteases other than Lon that are involved in the degradation of abnormal proteins.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference42 articles.

1. Adams M. H. 1959. Bacteriophages p. 445-447. John Wiley & Sons Inc. New York.

2. Analysis of a gene controlling cell division and sensitivity to radiation in Escherichia coli;Adler H. I.;J. Bacteriol.,1964

3. Mutants of Escherichia coli with a defect in the degradation of nonsense fragments;Bukhari A. I.;Nature (London) New Biol.,1973

4. Correlation between genetic and translational maps of gene 23 in bacteriophage T4;Celis J. E.;Nature (London) New Biol.,1973

5. ATP hydrolysis-dependent protease activity of the Ion (capR) protein of Escherichia coli K12;Charette M. F.;Proc. Natl. Acad. Sci. USA,1981

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3