Photoreversible Ultraviolet Enhancement of Infectivity in Agrobacterium tumefaciens

Author:

Heberlein Gary T.1,Lippincott James A.1

Affiliation:

1. Department of Biological Sciences, Northwestern University, Evanston, Illinois

Abstract

Heberlein, Gary T. (Northwestern University, Evanston, Ill.), and James A. Lippincott . Photoreversible ultraviolet enhancement of infectivity in Agrobacterium tumefaciens . J. Bacteriol. 89: 1511–1514. 1965.—Irradiation of a virulent strain of Agrobacterium tumefaciens with short-wavelength ultraviolet light, which reduces viability 50 to 90%, increases its infectivity as measured by the number of tumors initiated per viable bacterial cell on primary pinto bean leaves. A dark treatment of 1.5 to 2 hr between irradiation and inoculation results in a further increase in specific infectivity of as much as fourfold. This increase in infectivity is inhibited by white fluorescent light and light of 402 mμ, but only slightly by 549- or 619-mμ light. These results imply that some change in the deoxyribonucleic acid of the bacterium is responsible for this effect.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference5 articles.

1. Lysogenicity in Agrobacterium tumefaciens;BEARDSLEY RI.;J. Bacteriol.,1960

2. D)OUDNEY C. 0. 1962. UTltraviolet light-induced mutation as an event in the physiology of the bacterial cell p. 125-149. In F. H. Sobels [ed.I Repair from genetic radiation damage. Pergamon Press New York.

3. The irfluence of ultraviolet irradiation on the pathogenicity of Phytomonas tumefaciens;DUGGAR B. M.;Phvtopathology,1940

4. Studies on the virulence of bacteriophage infected strains of C. diptheriae;FREEMAN V'.;J. Bacteriol.,1951

5. HAYNES R. H. 1964. Molecular localizationi of radiation damage relevant to bacterial inactivation p. 51-68. In L. Augenstein R. Mason and B. Rosenberg [ed.] Physical processes in radiation biology. Academic Press Inc. New York.

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