Construction of Mobilizable Mini-Tn7Vectors for Bioluminescent Detection of Gram-Negative Bacteria and Single-Copy PromoterluxReporter Analysis-Reference-Cited by-同舟云学术

Construction of Mobilizable Mini-Tn7Vectors for Bioluminescent Detection of Gram-Negative Bacteria and Single-Copy PromoterluxReporter Analysis

Published:2013-04-12 Issue:13 Volume:79 Page:4149-4153
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl. Environ. Microbiol.

Author:

Damron F. Heath,McKenney Elizabeth S.,Barbier Mariette,Liechti George W.,Schweizer Herbert P.,Goldberg Joanna B.

Abstract

ABSTRACTWe describe the construction of mini-Tn7-based broad-host-range vectors encodingluxgenes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-luxintegration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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