Author:
Damron F. Heath,McKenney Elizabeth S.,Barbier Mariette,Liechti George W.,Schweizer Herbert P.,Goldberg Joanna B.
Abstract
ABSTRACTWe describe the construction of mini-Tn7-based broad-host-range vectors encodingluxgenes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-luxintegration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
53 articles.
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