Affiliation:
1. Departamento de Bioquímica, Escuela Nacional de Ciencias Biológicas, México, D.F.
Abstract
The lethal and mutagenic effects of H2O2 on wild-type Haemophilus influenzae Rd and on uvr1, uvr2, rec1, and rec2 mutant strains were studied. The first two mutants are sensitive to UV, and the second two are defective in recombination. Rd, urv1, and rec1 strains were more sensitive to the killing effect of H2O2 treatment than were uvr2 and rec2 strains. There were peaks of mutagenesis at two H2O2 concentrations over a range of 30 to 275 mM. Our results suggest a specific repair of H2O2 damage that is independent of the Uvr2 and Rec2 gene products. Sensitivity to the killing effect of H2O2 and to the lethal action of near-UV light were similar for Rd and uvr1 strains. This finding suggests that the mechanisms of killing by and repair of H2O2 damage may have some overlap with those of near-UV radiation.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference17 articles.
1. Characterization of an inducible oxidative stress system in Bacillus subtilis;Bol D. K.;J. Bacteriol.,1990
2. Black light;Cabrera-Juarez E.;J. Bacteriol.,1964
3. Nueva informaci 6n gendtica en el acido desoxirribonucleico por acci 6n directa de la Iuz cercana al ultravioleta;Chapital-Blanno L.;Rev. Latinoamer. Microbiol.,1985
4. Inducible repair of oxidative DNA damage in Escherichia coli;Demple B.;Nature (London),1983
5. Bacterial genes involved in response to near-ultraviolet radiation;Eisenstark A.;Adv. Genet.,1989
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献