Transposon Tn5supF-based reverse genetic method for mutational analysis of Escherichia coli with DNAs cloned in lambda phage-Reference-Cited by-同舟云学术

Transposon Tn5supF-based reverse genetic method for mutational analysis of Escherichia coli with DNAs cloned in lambda phage

Published:1991-01 Issue:2 Volume:173 Page:896-899
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Phadnis S H¹,Kulakauskas S¹,Krishnan B R¹,Hiemstra J¹,Berg D E¹

Affiliation:

1. Department of Molecular Microbiology, Washington University Medical School, St. Louis, Missouri 63110.

Abstract

An efficient method for systematic mutational analysis of the Escherichia coli genome was developed. It entails Tn5supF transposition to lambda-E. coli hybrid phage clones (Kohara library) and then transduction of recipient cells to Sup+. Essential and nonessential genes are distinguished by the ability of insertion mutant phage to form haploid versus only heterozygous partial diploid bacterial recombinants.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.173.2.896-899.1991

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