High correlation in antibody titers between the Sabin-Feldman dye test and an enzyme-linked immunosorbent assay detecting immunoglobulin G antibodies to the nucleoside triphosphate hydrolase of Toxoplasma gondii

Author:

Asai T1,Mizuno F1,Kojima S1,Takeuchi T1,Kobayashi A1,Suzuki Y1

Affiliation:

1. Department of Microbiology, Tokyo Medical College, Japan.

Abstract

An enzyme-linked immunosorbent assay to detect immunoglobulin G antibodies against nucleoside triphosphate hydrolase, which is a specific and dominant antigen of Toxoplasma gondii, was developed, and the sensitivity and specificity of the test were compared with those of the Sabin-Feldman dye test. One hundred percent agreement was observed in comparative study between those tests on 37 positive and 50 negative human sera. Antibody titers in the enzyme-linked immunosorbent assay test, which were expressed as the reciprocal of the highest positive dilution of serum, were just 100 times those in the dye test on 81% (30 of 37) of the positive sera.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference9 articles.

1. Do protozoa conceal a high potency of nucleoside triphosphate hydrolysis present in Toxoplasma gondii;Asai T.;Comp. Biochem. Physiol.,1986

2. Detection of nucleoside triphosphate hydrolase as a circulating antigen in sera of mice infected with Toxoplasma gondii;Asai T.;Infect. Immun.,1987

3. A potent nucleoside triphosphate hydrolase from the parasitic protozoan Toxoplasma gondii;Asai T.;J. Biol. Chem.,1983

4. Remarkable activities of nucleoside triphosphate hydrolase in the tachyzoites of both virulent and avirulent strains of Toxoplasma gondii;Asai T.;FEMS Microbiol. Lett.,1990

5. Role of serology in diagnosis of toxoplasmic;Brook R. G.;Iymphadenopathy. Rev. Infect. Dis.,1987

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