Immunomagnetic Separation Combined with Real-Time Reverse Transcriptase PCR Assays for Detection of Norovirus in Contaminated Food-Reference-Cited by-同舟云学术

Immunomagnetic Separation Combined with Real-Time Reverse Transcriptase PCR Assays for Detection of Norovirus in Contaminated Food

Published:2008-07 Issue:13 Volume:74 Page:4226-4230
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Park YoungBin¹,Cho You-Hee²,Jee YoungMee³,Ko GwangPyo¹⁴

Affiliation:

1. Department of Environmental Health and Institute of Health and Environment, School of Public Health, Seoul National University, Seoul, Korea

2. Department of Life Science, Sogang University, Seoul, Korea

3. Division of Enteric and Hepatitis Viruses, Center for Infectious Diseases, National Institute of Health, Korea Center for Disease Control and Prevention, Seoul, Korea

4. Institute of Microbiology, School of Biological Sciences, Seoul National University, Seoul, Korea

Abstract

ABSTRACT We developed an immunomagnetic separation (IMS) technique combined with real-time TaqMan reverse transcriptase PCR (RT-PCR), which allowed detection of norovirus at a level as low as 3 to 7 RT-PCR units from artificially contaminated strawberries. The inoculum recovery rate ranged from 14 to 30%. The data demonstrate that IMS combined with real-time RT-PCR will be useful as a rapid and sensitive method for detecting food-borne microbial contaminants.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00013-08

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