Ribosome Maturation of Escherichia coli Growing at Different Growth Rates

Abstract

The data reported here are consistent with the hypothesis that the rate of ribosome assembly in vivo approximates a constant fraction of the generation time for the four rates studied. This conclusion is indicated by the following. (i) There is an increased lag period before radioisotopically labeled uracil appears in 23 and 16 S ribosomal ribonucleic acid of 70 S ribosomes as a function of growth rate. (ii) The time necessary for 3 H-uracil in the 43 S ribonucleoprotein precursor to the 50 S subunit to assume a position at 50 S in sucrose gradients is greatly increased inversely to the growth rate.