Development and Application of a Novel Peptide Nucleic Acid Probe for the Specific Detection of Helicobacter pylori in Gastric Biopsy Specimens-Reference-Cited by-同舟云学术

Development and Application of a Novel Peptide Nucleic Acid Probe for the Specific Detection of Helicobacter pylori in Gastric Biopsy Specimens

Published:2007-09 Issue:9 Volume:45 Page:3089-3094
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Guimarães N.¹²,Azevedo N. F.¹³,Figueiredo C.²⁴,Keevil C. W.³,Vieira M. J.¹

Affiliation:

1. IBB (Institute for Biotechnology and Bioengineering), Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar 4710-057, Braga

2. IPATIMUP (Institute of Molecular Pathology and Immunology of the University of Porto)

3. School of Biological Sciences, University of Southampton, Southampton, United Kingdom

4. Medical Faculty of Porto, Porto, Portugal

Abstract

ABSTRACT In this work, a fluorescence in situ hybridization (FISH) method for the rapid detection of Helicobacter pylori using a novel peptide nucleic acid (PNA) probe is reported. Laboratory testing with several different bacterial species, including other Helicobacter spp., has shown that this probe is highly specific for H. pylori strains. In addition, the PNA FISH method has been successfully adapted for detection of the pathogen in paraffin-embedded gastric biopsy specimens.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00858-07

Reference34 articles.

1. Andersen, L. P., and T. Wadstrom. 2001. Basic bacteriology and culture, p. 27-38. In H. L. T. Mobley, G. L. Mendz, and S. L. Hazell (ed.), Helicobacter pylori: physiology and genetics. ASM Press, Washington, DC.

2. New Screening Software Shows that Most Recent Large 16S rRNA Gene Clone Libraries Contain Chimeras

3. Ashelford, K. E., A. J. Weightman, and J. C. Fry. 2002. PRIMROSE: a computer program for generating and estimating the phylogenetic range of 16S rRNA oligonucleotide probes and primers in conjunction with the RDP-II database. Nucleic Acids Res.30:3481-3489.

4. Azevedo, N. F., M. J. Vieira, and C. W. Keevil. 2003. Development of peptide nucleic acid probes to detect Helicobacter pylori in diverse species potable water biofilms, p. 105-112. In A. McBain, D. Allison, M. Brading, A. Rickard, J. Verran, and J. Walker (ed.), Biofilm communities: order from chaos? BioLine, Cardiff, United Kingdom.

5. Azevedo, N. F., M. J. Vieira, and C. W. Keevil. 2003. Establishment of a continuous model system to study Helicobacter pylori survival in potable water biofilms. Water Sci. Technol.47:155-160.

Cited by 52 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Oligonucleotide probes for imaging and diagnosis of bacterial infections;Critical Reviews in Biotechnology;2024-06-03

2. In Search for Reasons behind Helicobacter pylori Eradication Failure–Assessment of the Antibiotics Resistance Rate and Co-Existence of Helicobacter pylori with Candida Species;Journal of Fungi;2023-03-07

3. Emerging Technologies to Investigate the Potential of Gut Microbiota in Human Health;Role of Microbes in Sustainable Development;2023

4. The role of Nucleic Acid Mimics (NAMs) on FISH-based techniques and applications for microbial detection;Microbiological Research;2022-09

5. Application of Nucleic Acid Mimics in Fluorescence In Situ Hybridization;Methods in Molecular Biology;2021