Development and Application of a Novel Peptide Nucleic Acid Probe for the Specific Detection of Helicobacter pylori in Gastric Biopsy Specimens-Reference-Cited by-同舟云学术

Development and Application of a Novel Peptide Nucleic Acid Probe for the Specific Detection of Helicobacter pylori in Gastric Biopsy Specimens

Published:2007-09 Issue:9 Volume:45 Page:3089-3094
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Guimarães N.¹²,Azevedo N. F.¹³,Figueiredo C.²⁴,Keevil C. W.³,Vieira M. J.¹

Affiliation:

1. IBB (Institute for Biotechnology and Bioengineering), Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar 4710-057, Braga

2. IPATIMUP (Institute of Molecular Pathology and Immunology of the University of Porto)

3. School of Biological Sciences, University of Southampton, Southampton, United Kingdom

4. Medical Faculty of Porto, Porto, Portugal

Abstract

ABSTRACT In this work, a fluorescence in situ hybridization (FISH) method for the rapid detection of Helicobacter pylori using a novel peptide nucleic acid (PNA) probe is reported. Laboratory testing with several different bacterial species, including other Helicobacter spp., has shown that this probe is highly specific for H. pylori strains. In addition, the PNA FISH method has been successfully adapted for detection of the pathogen in paraffin-embedded gastric biopsy specimens.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00858-07

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