Detection of latent cytomegalovirus in murine salivary and prostate explant cultures and cells

Abstract

After infection of adult mice, cytomegalovirus was detectable in salivary gland suspensions by tissue culture inoculation for up to 3 months. After these cultures had become negative, virus apparently latent in these tissues could be detected in explants of salivary and prostate glands and in cell lines derived from these explants. In some cases cycles of virus-induced cell injury and regrowth were observed. Murine cytomegalovirus plaque efficiency and morphology were evaluated in prostate and salivary gland cell cultures derived from previously infected and uninfected mice. No evidence of interference was detected, although plaques size was altered (larger) in prostate cells from previously infected mice. These studies indicate the presence of a range of suppression, latency, or effects of murine cytomegalovirus detectable after the resolution of active infection and provide methods for additional study of the establishment and activation of virus latency.