Pseudomonas aeruginosa Interstrain Dynamics and Selection of Hyperbiofilm Mutants during a Chronic Infection

Author:

Gloag Erin S.1,Marshall Christopher W.23,Snyder Daniel23,Lewin Gina R.45,Harris Jacob S.1,Santos-Lopez Alfonso23,Chaney Sarah B.1,Whiteley Marvin45,Cooper Vaughn S.23ORCID,Wozniak Daniel J.16

Affiliation:

1. Department of Microbial Infection and Immunity, The Ohio State University, Columbus, Ohio, USA

2. Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA

3. Center for Evolutionary Biology and Medicine, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA

4. School of Biological Sciences, Georgia Institute of Technology, Atlanta, Georgia, USA

5. Emory-Children’s Cystic Fibrosis Center, Atlanta, Georgia, USA

6. Department of Microbiology, The Ohio State University, Columbus, Ohio, USA

Abstract

Bacteria adapt to infections by evolving variants that are more fit and persistent. These recalcitrant variants are typically observed in chronic infections. However, it is unclear when and why these variants evolve. To address these questions, we used a porcine chronic wound model to study the evolutionary dynamics of Pseudomonas aeruginosa in a mixed-strain infection. We isolated hyperbiofilm variants that persisted early in the infection. Interstrain interactions were also observed, where adapted variants acquired CRISPR-mediated immunity to phages. We show that when initiating infection, P. aeruginosa experiences strong positive selection for hyperbiofilm phenotypes produced by mutants of a single chemosensory system, the Wsp pathway. We predict that hyperbiofilm variants are early adaptations to infection and that interstrain interactions may influence bacterial burden and infection outcomes.

Funder

HHS | National Institutes of Health

Cystic Fibrosis Foundation

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

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