Affiliation:
1. Department of Clinical Laboratory Medicine
2. Division of Laboratory Diagnosis, Sapporo Medical University School of Medicine, Sapporo, Japan
Abstract
ABSTRACT
We investigated the significance of endogenous reactive oxygen species (ROS) produced by fungi treated with miconazole. ROS production in
Candida albicans
was measured by a real-time fluorogenic assay. The level of ROS production was increased by miconazole at the MIC (0.125 μg/ml) and was enhanced further in a dose-dependent manner, with a fourfold increase detected when miconazole was used at 12.5 μg/ml. This increase in the level of ROS production was completely inhibited by pyrrolidinedithiocarbamate (PDTC), an antioxidant, at 10 μM. In a colony formation assay, the decrease in cell viability associated with miconazole treatment was significantly prevented by addition of PDTC. Moreover, the level of ROS production by 10 clinical isolates of
Candida
species was inversely correlated with the miconazole MIC (
r
= −0.8818;
P
< 0.01). These results indicate that ROS production is important to the antifungal activity of miconazole.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Cited by
281 articles.
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