Affiliation:
1. U.S. Food and Drug Administration Center for Veterinary Medicine, Laurel, Maryland 20708
2. Department of Nutrition and Food Science, University of Maryland, College Park, Maryland 20742
Abstract
ABSTRACT
Sixteen isolates of
Enterococcus faecalis
were recovered from retail poultry samples (seven chickens and nine turkeys) purchased from grocery stores in the greater Washington, D.C., area. PCR for known streptogramin resistance genes identified
vat
(E) in five
E. faecalis
isolates (three isolates from chickens and two isolates from turkeys). The
vat
(E) gene was transmissible on a ca. 70-kb plasmid, along with resistance to erythromycin, tetracycline, and streptomycin, by conjugation to
E. faecalis
and
Enterococcus faecium
recipient strains. DNA sequencing showed little variation between
E. faecalis vat
(E) genes from the chicken samples; however, one
E. faecalis vat
(E) gene from a turkey sample possessed 5 nucleotide changes that resulted in four amino acid substitutions. None of these substitutions in the
vat
(E) allele have previously been described. This is the first report of
vat
(E) in
E. faecalis
and its transferability to
E. faecium
, which indicates that
E. faecalis
can act as a reservoir for the dissemination of
vat
(E)-mediated streptogramin resistance to
E. faecium
.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Cited by
27 articles.
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