Affiliation:
1. URA INRA-DGER d’Immunopathologie Cellulaire et Moléculaire, Ecole Nationale Vétérinaire d’Alfort, 94704 Maisons-Alfort Cedex, France
Abstract
ABSTRACT
In this study, we show that bovine leukemia virus (BLV)-induced persistent lymphocytosis (PL) results from the in vivo expansion of the CD11b
+
B-lymphocyte population. This subset shares phenotypic characteristics with murine and human B-1 cells. BLV interactions with the sheep B-1-like subset were explored. We found that B-1- and B-2-like cells are initially infected to similar extents. However, in long-term-infected sheep, the viral load is higher in B-1-like cells and only B-1- and not B-2-like cells show increased ex vivo survival compared to that in uninfected sheep. Ex vivo viral expression was found in both B-1- and B-2-like cells, indicating that both cell types support viral replication. Finally, cycloheximide and a protein kinase C inhibitor (H7) that blocks the ex vivo activation of viral expression did not affect the increased survival in B-1-like cells, suggesting that resistance to apoptosis is acquired in vivo. Collectively, these results indicate a peculiar susceptibility of sheep B-1-like cells to BLV transforming effects and further support the involvement of increased survival in BLV pathogenesis.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference34 articles.
1. Association of GP51 expression and persistent CD5+ B-lymphocyte expansion with lymphomatogenesis in bovine leukemia virus infected sheep;Birkebak T. A.;Leukemia,1994
2. Repression of bax gene expression by the HTLV-1 Tax protein: implications for suppression of apoptosis in virally infected cells;Brauweiler A.;Virology,1997
3. Burny
A.
Bruck
C.
Chanhenne
H.
Cleuter
Y.
Dekegel
D.
Ghysdael
J.
Kettmann
R.
Leclercq
M.
Leunen
J.
Mammerickx
M.
Portetelle
D.
Bovine leukemia virus: molecular biology and epidemiology
1980
231
278
Raven Press
New York N.Y
4. Treatment of murine CD5− B-cells with anti-Ig, but not LPS, induces surface CD5: two B-cell activation pathways;Cong Y.-Z.;Int. Immunol.,1991
5. Induction of tumor necrosis factor alpha in human neuronal cells by extracellular human T-cell lymphotropic virus type 1 Tax
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