Effect of inoculum size on the phenotypic characterization of Campylobacter species

Abstract

The type strains of six Campylobacter species or subspecies were examined in eight tests used for the identification of such organisms. False-positive results were obtained in certain tests (growth on 1% bile, brilliant green, selenite, trimethylamine-N-oxide, 2,3,5-triphenyl tetrazolium chloride, and minimal media) when an inoculum yielding 10(7) to 10(8) CFU/ml was used. Each tolerance test was examined with blood, nutrient, and brucella agars as basal media. The type of basal medium used could also affect the test outcome. With the inoculum standardized to a density yielding approximately 10(6) CFU/ml, reproducible and pertinent results were obtained, provided an appropriate basal medium was used. However, 95% confidence limits for viable counts done on these basal media indicated that blood agar may yield more consistent results than the other agars examined.