Affiliation:
1. Division of Biology, Kansas State University, Manhattan 66506.
Abstract
Oocysts of Cryptosporidium parvum were used to infect Madin-Darby bovine kidney cells. Cultures were incubated in a reduced-oxygen atmosphere in candle jars or in a 5% CO2-95% air atmosphere. At 72 h, parasites were quantitated microscopically and found to be enhanced 5.5-fold in the reduced-oxygen atmosphere. Using candle jars, we then determined that C. parvum was amenable to [3H]uracil incorporation assays and easily quantitated with this method.
Publisher
American Society for Microbiology
Reference24 articles.
1. Mise au point d'un modele experimental de culture in vitro des stades asexues de Cryptosporidium sp;Bonnin A.;Ann. Parasitol. Hum. Comp.,1990
2. Current W. L. 1990. Techniques and laboratory maintenance of Cryptosporidium p. 31-49. In J. P. Dubey C. A. Speer and R. Fayer (ed.) Cryptosporidiosis of man and animals. CRC Press Inc. Boca Raton Fla.
3. Complete development of Cryptosporidium in cell culture;Current W. L.;Science,1984
4. A comparison of endogenous development of three isolates of Cryptosporidium in suckling mice;Current W. L.;J. Protozool.,1986
5. Developpement complet de Cryptosporidium en culture cellulaire: applications;Datry A.;Med. Sci.,1989
Cited by
17 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献