Enzymatic determination of itoic acid, a Bacillus subtilis siderophore, and 2,3-dihydroxybenzoic acid

Author:

Ito T1

Affiliation:

1. Department of Biology, East Carolina University, Greenville, North Carolina 27858-4353.

Abstract

A specific enzymatic method to determine the amounts of itoic acid, a Bacillus subtilis siderophore, and 2,3-dihydroxybenzoic acid (2,3-DHBA) was devised. A sample was incubated first with hippurate hydrolase and then with 2,3-DHBA-3,4-dioxygenase. Itoic acid was estimated from the increase in A374. The incubation with the first enzyme was omitted for the determination of 2,3-DHBA.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference16 articles.

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2. Dagley S. W. C. Evans and D. W. Ribbons. 1960. New NOTES 2345

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4. Lunar soil and Zagami meteorite inhibit biosynthesis of itoic acid, a siderophore;Ito T.;Experientia,1987

5. .Ito T. Unpublished observations.

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