Affiliation:
1. Division of Biotechnology, Centre for Applied Microbiology and Research, Salisbury, Wiltshire, United Kingdom.
Abstract
Bacillus cereus SM3 was isolated on a mineral salts medium with Tween 80 as the primary carbon source. It was able to hydrolyze second- and third-generation pyrethroids, thereby generating noninsecticidal products. The enzyme responsible for this hydrolytic reaction was named permethrinase for this study. This is the first instance in which pyrethroid detoxification has been achieved with a cell-free microbial enzyme system. Permethrinase was purified by ion-exchange chromatography and gel filtration chromatography. The molecular mass of native permethrinase was 61 +/- 3 kDa, as estimated by Sephadex G-100 gel filtration. This novel microbial esterase seems to be a carboxylesterase. Permethrinase activity had an optimum pH of 7.5 and a temperature optimum of 37 degrees C. No cofactors or coenzymes were required for permethrinase activity. The enzyme may be a serine esterase, as it seems to be sensitive to the organophosphorus compound tetraethylpyrophosphate at concentrations in the micromolar range. Addition of dithiothreitol afforded permethrinase protection against the inhibitory effects of the sulfydryl agents p-chloromercuribenzoate and N-ethylmaleimide. The enzyme was stable over a range of temperatures. Cell extracts of strain SM3 also contained another esterase, which was active towards beta-naphthylacetate, but this enzyme was distinct from permethrinase.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference44 articles.
1. Physical properties and kinetic behavior of a cephalosporin acetylesterase produced by Bacillus subtilis;Abbott B. J.;Appl. Microbiol.,1975
2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding;Bradford M. M.;Anal. Biochem.,1976
3. -1976. Structure-biodegradability relationships in pyrethroid insecticides;Casida J. E.;Arch. Environ. Contam. Toxicol.,1975
4. An introduction to the metabolism of pyrethroids;Chambers J.;Residue Rev.,1980
5. Chiaki S. K. Nishizawa M. Sugimoto K. Sonada and F. Kishimoto. 1987. A novel microorganism a novel esterase and method for preparing the same. European patent application 024 3167 Sumitomo Chemical Company Ltd.
Cited by
47 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献