Regulation of RraA, a Protein Inhibitor of RNase E-Mediated RNA Decay-Reference-Cited by-同舟云学术

Regulation of RraA, a Protein Inhibitor of RNase E-Mediated RNA Decay

Published:2006-05 Issue:9 Volume:188 Page:3257-3263
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Zhao Meng¹,Zhou Li²,Kawarasaki Yasuaki¹³,Georgiou George¹³⁴

Affiliation:

1. Institute for Cell and Molecular Biology, University of Texas at Austin, Austin, Texas 78712

2. Graduate Program in Microbiology, University of Texas at Austin, Austin, Texas 78712

3. Department of Chemical Engineering, University of Texas at Austin, Austin, Texas 78712

4. Department of Biomedical Engineering, University of Texas at Austin, Austin, Texas 78712

Abstract

ABSTRACT The recently discovered RraA protein acts as an inhibitor of the essential endoribonuclease RNase E, and we demonstrated that ectopic expression of RraA affects the abundance of more than 700 transcripts in Escherichia coli (K. Lee, X. Zhan, J. Gao, J. Qiu, Y. Feng, R. Meganathan, S. N. Cohen, and G. Georgiou, Cell 114:623-634, 2003). We show that rraA is expressed from its own promoter, P rraA , located in the menA-rraA intergenic region. Primer extension and lacZ fusion analysis revealed that transcription from P rraA is elevated upon entry into stationary phase in a σ s -dependent manner. In addition, the stability of the rraA transcript is dependent on RNase E activity, suggesting the involvement of a feedback circuit in the regulation of the RraA level in E. coli .

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.188.9.3257-3263.2006

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