Affiliation:
1. Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-0308
Abstract
ABSTRACT
Archaea have been shown to produce isoprenoids from mevalonate; however, genome analysis has failed to identify several genes in the mevalonate pathway on the basis of sequence similarity. A predicted archaeal kinase, coded for by the MJ0044 gene, was associated with other mevalonate pathway genes in the archaea and was predicted to be the “missing” phosphomevalonate kinase. The MJ0044-derived protein was tested for phosphomevalonate kinase activity and was found not to catalyze this reaction. The MJ0044 gene product was found to phosphorylate isopentenyl phosphate, generating isopentenyl diphosphate. Unlike other known kinases associated with isoprene biosynthesis,
Methanocaldococcus jannaschii
isopentenyl phosphate kinase is predicted to be a member of the aspartokinase superfamily.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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