Affiliation:
1. Institute of Biochemistry, National Yang-Ming Medical College, Taipei, Taiwan 11221, Republic of China
Abstract
A
d
-aminoacylase-producing microorganism, strain DA181, isolated from soil was identified as
Alcaligenes denitrificans
subsp.
denitrificans
. This strain produced about 29,300 units (micromoles of product formed per hour) of
d
-aminoacylase and 2,300 units of
l
-aminoacylase per gram of cells (wet weight) when cultivated in a medium containing 1%
N
-acetyl-
dl
-leucine as the carbon source. The
d
-aminoacylase was purified 345-fold. The specific activity of the purified enzyme was 108,600 units per mg of protein when
N
-acetyl-
d
-methionine was used as a substrate. The apparent molecular weight was 58,000, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
N
-Acetyl-
d
-methionine was the favored substrate, followed by
N
-acetyl-
d
-phenylalanine. This enzyme had a high stereospecificity, and its hydrolysis of
N
-acetyl-
l
-amino acids was almost negligible.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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