Affiliation:
1. Department of Microbiology, Saint Louis University School of Medicine, Saint Louis, Missouri 63104
Abstract
The kinetics of degradation of bacterial deoxyribonucleic acid (DNA) after infection of
Escherichia coli
with T4D, ultraviolet-irradiated T4D, and two amber mutants, N122 and N94, was studied by zone sedimentation through linear glycerol gradients. Within 5 min after infection with any of the bacteriophages, breakdown of host genome was evident. The first product was a high-molecular-weight material (50
S
to 70
S
) and further degradation appeared to occur in discrete steps. Rapid and extensive breakdown of bacterial DNA was seen after infection with
am
N122 and T4D. Infection with ultraviolet-irradiated phage or with
am
N94 resulted in an accumulation of high-molecular-weight material. These results suggest that the observed degradation of host DNA begins early and requires sequential action of several phage-induced endo- as well as exodeoxyribonucleases.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference23 articles.
1. Induced synthesis of enzymes in bacteria analyzed at the cellular level;BENZER S.;Biochim. Biophys. Acta,1958
2. On the stability of phage messenger RNA;BOSE S. K.;Biochem. Biophys. Res. Commun.,1967
3. In vivo stability of phage messenger ribonucleic acid;BOSE S. K.;Proc. Natl. Acad. U.,1967
4. The synthesis of bacterial viruses. I. The synthesis of nucleic acid and protein in E. coli B infected with T2r+ bacteriophage;COHEN S. S.;J. Biol. Chem.,1948
5. Effect of ultraviolet irradiation of bacteriophage T2 on enzyme synthesis in host cells;DIRKSEN M.-L.;Proc. Natl. Acad. Sci. U.,1960
Cited by
43 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献